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Stable Transfection

Transfection

Introducing DNA into eukaryotic cells, such as animal cells, is called transfection. Transfection typically involves opening transient "holes" or gates in cells to allow the entry of extracellular molecules, typically supercoiled plasmid DNA, but also siRNA, among others. Several different transfection techniques are available, including calcium phosphate transfection, lipofection, electroporation, heat shock, and proprietary transfection reagents such as Fugene. The choice of transfection methods differs upon cell sensitivity to the conditions, and the amount of work and cost involved. Typically, transfection of bacteria is done by heat shock or electroporation, which are quick, cheap and easy procedures to carry out, as bacteria are easy to grow and multiply rapidly, even from a small portion of survivors. For eukaryotic cells, lipid- cation based transfection is more typically used, as the cells are more sensitive. The drawback is that this is costlier, and requires more work, which is typical of eukaryotic cell culture procedures in general. DNA can also be introduced into cells using viruses as a carrier. In such cases, the technique is called viral transduction, and the cells are said to be transduced. category:Molecular biology pl:Transfekcja

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Come To The Stable

''Come to the Stable'' is a 1949 film which tells the story of two French nuns who come to a small New England town and involve the townsfolk in helping them to build a children's hospital. It stars Loretta Young, Celeste Holm, Hugh Marlowe, Elsa Lanchester, Thomas Gomez, Dooley Wilson and Regis Toomey. The movie was written by Sally Benson, Clare Boothe Luce and Oscar Millard. It was directed by Henry Koster. It was nominated for Academy Awards for Best Actress in a Leading Role (Loretta Young), Best Actress in a Supporting Role (Celeste Holm), Best Actress in a Supporting Role (Elsa Lanchester), Best Art Direction-Set Decoration, Black-and-White, Best Cinematography, Best Music, Song (Alfred Newman and Mack Gordon for "Through a Long and Sleepless Night") and Best Writing, Motion Picture Story.

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Category:1949 films Category:Best Actress Oscar Nominee (film) Category:Best Supporting Actress Oscar Nominee (film) Category:Best Song Oscar Nominee

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Stable

:''This article is about the building; for another meaning, see stability.'' A stable is a building in which livestock, usually horses, are kept. It most commonly means a building that is divided into separate stalls for individual animals. Used metaphorically from this origin, a stable is a collection of people (i.e. professional wrestlers) working under a single manager or animals kept by one owner.

Barn Category:Buildings and structures

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Stable Isotope

Different isotopes of one chemical element may have different radioactive properties. Those isotopes that are not radioactive are called stable. While stable isotopes of the same element maintain the same chemical characteristics and therefore react in the same way, the mass difference as a result of an extra few neutrons results in partial separation of the light from heavy isotopes during chemical reactions (isotopic fractionation). For example, the difference in mass between the two main isotopes of hydrogen H-1 (1 proton, no neutron) and H-2 (also known as deuterium; 1 proton, 1 neutron) is almost 100%. Therefore, a significant fractionation will occur. Commonly analysed stable isotopes include oxygen, carbon, hydrogen and sulfur. These isotope systems have been under investigation for many years as they are relatively simple to measure. Recent advances in mass spectrometry (ie: muliple-collector inductively coupled plasma mass spectrometry) now enable the measurement of heavier stable isotopes, such as iron, copper, zinc, molybdenum, etc.

Stable isotope fractionation

There are three types of isotope fractionation:

equilibrium fractionation

kinetic fractionation

mass-independent fractionation

isotope table (complete)

isotope table (divided)

eo:Stabila izotopo es:Isótopo estable

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Stable Transfection